Liposome-Based Silibinin for Mitigating Nonalcoholic Fatty Liver Disease: Dual Effects via Parenteral and Intestinal Routes.

Nonalcoholic fatty liver disease (NAFLD) is a clinicopathological entity that is typically characterized by intrahepatic ectopic steatosis. Nowadays, NAFLD has surpassed viral hepatitis and become the most common chronic liver disease worldwide, which poses a great threat to human health. Silibinin (Sil), a well-known natural product, has been widely used in clinical treatment for liver disorders and exhibited therapeutic potential for NAFLD. However, the suitability of Sil for NAFLD treatment still requires further investigation due to its limited absorption and low bioavailability. This study aimed to construct a Sil-loaded liposome (Sil-Lip) to overcome the limitations of Sil, thereby enhancing its beneficial effects on NAFLD and then investigate the underlying mechanisms of action of Sil-Lip. Herein, Sil-Lip was fabricated by a well-established thin-film dispersion method and carefully characterized, followed by evaluating their therapeutic efficacy using high-fat diet-induced NAFLD mice and free fatty acid -stimulated HepG2 cells. Then, liver transcriptome analysis and 16S ribosomal RNA (16S rRNA) sequencing were utilized to elucidate the potential mechanisms of action of Sil-Lip. Our data indicated that Sil-Lip harbored good gastrointestinal tract stability, mucus layer permeation, and excellent oral absorption and bioavailability. In vivo and in vitro NAFLD models demonstrated that Sil-Lip had better effects in alleviating lipid metabolism disorders, insulin resistance, and inflammation than did Sil alone. Further investigations revealed that the beneficial effects of Sil-Lip were mediated by modulating intrahepatic insulin resistance-related and nuclear factor-kappa B (NF-κB) signaling pathways and extrahepatic gut microbiota. Our study confirmed that Sil-Lip can effectively improve the absorption and bioavailability of Sil, resultantly potentiating its ameliorative effects on NAFLD through modulating intrahepatic insulin resistance-related and NF-κB signaling pathways and extrahepatic gut microbiota.

In situ gelling hydrogel loaded with berberine liposome for the treatment of biofilm-infected wounds.

Background: In recent years, the impact of bacterial biofilms on traumatic wounds and the means to combat them have become a major research topic in the field of medicine. The eradication of biofilms formed by bacterial infections in wounds has always been a huge challenge. Herein, we developed a hydrogel with the active ingredient berberine hydrochloride liposomes to disrupt the biofilm and thereby accelerate the healing of infected wounds in mice. Methods: We determined the ability of berberine hydrochloride liposomes to eradicate the biofilm by means of studies such as crystalline violet staining, measuring the inhibition circle, and dilution coating plate method. Encouraged by the in vitro efficacy, we chose to coat the berberine hydrochloride liposomes on the Poloxamer range of in-situ thermosensitive hydrogels to allow fuller contact with the wound surface and sustained efficacy. Eventually, relevant pathological and immunological analyses were carried out on wound tissue from mice treated for 14 days. Results: The final results show that the number of wound tissue biofilms decreases abruptly after treatment and that the various inflammatory factors in them are significantly reduced within a short period. In the meantime, the number of collagen fibers in the treated wound tissue, as well as the proteins involved in healing in the wound tissue, showed significant differences compared to the model group. Conclusion: From the results, we found that berberine liposome gel can accelerate wound healing in Staphylococcus aureus infections by inhibiting the inflammatory response and promoting re-epithelialization as well as vascular regeneration. Our work exemplifies the efficacy of liposomal isolation of toxins. This innovative antimicrobial strategy opens up new perspectives for tackling drug resistance and fighting wound infections.

Treatment of Idiopathic Pulmonary Fibrosis by Inhaled Silybin Dry Powder Prepared via the Nanosuspension Spray Drying Technology.

Idiopathic pulmonary fibrosis (IPF) is a kind of life-threatening interstitial lung disease characterized by progressive dyspnea with accurate pathogenesis unknown. At present, heat shock protein inhibitors are gradually used to treat IPF. Silybin, a heat shock protein C-terminal inhibitor, has high safety and good application prospects. In this work, we have developed a silybin powder able to be used for inhalation administration for the treatment of IPF. Silybin powder was prepared by the spray drying method and identified using cascade impactometry, particle size, scanning electron microscopy (SEM), differential scanning calorimetry (DSC), X-ray diffraction (XRD), and Fourier transform infrared (FT-IR) spectroscopy. A rat model of bleomycin-induced IPF was used to assess the effect of inhaled silybin spray-dried powder. Lung hydroxyproline content, wet weight, histology, inflammatory factor expression, and gene expression were examined. The results showed that inhaled silybin spray-dried powder alleviated inflammation and fibrosis, limited hydroxyproline accumulation in the lungs, modulated gene expression in the development of IPF, and improved postoperative survival. The results of this study suggest that silybin spray-dried powder is an attractive candidate for the treatment of IPF.

Green mergers and acquisitions and green innovation: an empirical study on heavily polluting enterprises.

The concept of green development has gradually penetrated into the enterprise. Green mergers and acquisitions (M&A) have gradually become a means for heavily polluting enterprises to achieve the goal of energy conservation and emission reduction and embark on the path of green transformation. Heavily polluting enterprises have acquired clean technology and resources through green M&A, and whether they will promote their green innovations after green M&A has not yet been explored. Based on the data of M&As of China's heavy polluting enterprises from 2010 to 2018, this study empirically tests whether the M&As of heavy polluting enterprises can promote green innovation. The results show that M&As by heavily polluting enterprises can promote green innovation, and this impact is promoted with the support of government subsidies. In addition, older or higher paid CEOs negatively moderate this effect. Therefore, our study believes that most of the M&As of heavy polluting enterprises are taking the initiative to take environmental protection responsibilities and embarking on the path of green transformation. The government can issue relevant policies to encourage heavily polluting enterprises to conduct green M&A in order to achieve their goal of green transformation. Our study has enriched the relevant literature on green investment and green innovation, and can be used as a reference for the government to introduce policies for the green transformation of heavily polluting enterprises.

Cholesterol Microdomain Enhances the Biofilm Eradication of Antibiotic Liposomes.

Resistance and tolerance of biofilms to antibiotics is the greatest challenge in the treatment of bacterial infections. Therefore, developing an effective strategy against biofilms is a top priority. Liposomes are widely used as antibiotic drug carriers; however, common liposomes lack affinity for biofilms. Herein, biofilm-targeted antibiotic liposomes are created by simply adjusting their cholesterol content. The tailored liposomes exhibit significantly enhanced bacterial inhibition and biofilm eradication effects that are positively correlated with the cholesterol content of liposomes. The experiments further demonstrate that this enhanced effect can be ascribed to the effective drug release through the pores, which are formed by the combination of cholesterol microdomains in liposomal lipid bilayers with membrane-damaged toxins in biofilms. Consequently, liposome encapsulation with a high cholesterol concentration improves noticeably the pharmacodynamics and biocompatibility of antibiotics after pulmonary administration. This work may provide a new direction for the development of antibiofilm formulations that can be widely used for the treatment of infections caused by bacterial biofilms.

Don't Speak Ill of Others Behind Their Backs: Receivers' Ostracism (Sender-Oriented) Reactions to Negative Workplace Gossip.

PURPOSE: Previous studies on negative workplace gossip have neglected the role of gossip receivers. The current study aims to explore the interpersonal interaction mechanism between gossip receivers and communicators. Drawing on social information processing theory, we propose a theoretical model for the relationships between negative workplace gossip, psychological safety, ostracism, and coworker-exchanges. PARTICIPANTS AND METHODS: Multi-wave data of 386 employees from eight service-oriented companies in China supported the proposed framework. Critical incident techniques and time-lag method were used for data collection. SPSS and Mplus were employed for hypothesis test. RESULTS: The empirical results indicated that negative workplace gossip was positively related to gossip receivers' ostracism (sender-oriented), among which receivers' psychological safety (sender-oriented) played a mediating role. In addition, the coworker exchange relationship moderated the relationship between negative workplace gossip and ostracism, and the psychological safety of interviewees also plays a mediating role. Specifically, the effect of negative workplace gossip on psychological safety and the mediating effect of psychological safety were stronger when the coworker exchange relationship was higher. CONCLUSION: Drawing on social information processing theory, the present study constructed a process model of the recipients' sender-oriented ostracism reactions to negative workplace gossip, which helps explain the cognitive psychological mechanism and the boundary conditions of the above "perception-interpretation-behavior" social information process model. The above framework contributes to both theory and practice.

Cyberloafing to Escape From the "Devil": Investigating the Impact of Abusive Supervision From the Third-Party Perspective.

PURPOSE: Previous studies on cyberloafing focus on individual and organization factors, ignoring the situation of employes as the event observers. Drawing on affective events theory (AET), the present study proposed a theoretical model for the relationships among peer abusive supervision, negative affectivity, cyberloafing, and hostile attribute bias, which aims to bridge the above research gap. METHODOLOGY: Multiwave data of 355 employes from 8 service-oriented companies in Southwest China supported our model. Time-lag method and critical incident techniques were introduced during the data collection stage. Ordinary least squares (OLS) regression and bootstrapping method were employed for hypothesis test. FINDINGS: The empirical results indicated that peer abusive supervision was positively related to third party's cyberloafing, and the third party's negative affectivity plays a mediating role among the above relationships. In addition, the third party's hostile attribution bias moderated the mediating role of third party's negative affectivity. Specifically, the effect of peer abusive supervision on third party's negative affectivity and the mediating effect of this negative affectivity were stronger when the third party's hostile attribution bias was higher. ORIGINALITY: Drawing on AET, the current study constructed a process model of third party's cyberloafing reactions to peer abusive supervision, which helps explain the affective mechanism and the boundary conditions of the above "events-affectivity-behavior" path. Our model is a positive response to previous scholars' calls for research of abusive supervision from multiple perspectives. Meanwhile, the current study explored the antecedent variable of cyberloafing from the perspective of event observers, which provides a theoretical basis for follow-up-related research. Thirdly, this study further expanded the theoretical boundaries of AET.

Analysis of the Agricultural Economy and Agricultural Pollution Using the Decoupling Index in Chengdu, China.

The development of the agricultural economy has brought the agricultural pollution, and agricultural pollution has restrained the growth of the agricultural economy and endangered public health. Since the Economic Reform opened up, China has promulgated many policies that have benefited farmers, developed the agricultural economy, and improved agricultural material levels. However, the growth in the agricultural economy has also resulted in significant environmental pollution from pesticides, agricultural fertilizers and agricultural plastic film. Agricultural pollution can affect public health, therefore it is necessary to study the relationship between agricultural economy and agricultural pollution. In this paper, the relationship between the agricultural economy and agricultural pollution is studied by using decoupling index method, taking Chengdu, Sichuan Province, China as an example, and verified by the data of 2008-2017 years of Chengdu. It was found that the decoupling index for pesticide use and per capita gross agricultural production showed "strong decoupling" ideal states in six years. From 2009-2017, the value of it increased from 0.065743-1.597385, and its decoupling state gradually transited to stable strong decoupling. The chemical fertilizer use was found to have a "strong decoupling" ideal state in five years. The value of it decreased from 1.028854-(-0.512702), while its state gradually changed to strong decoupling. However, agricultural plastic film only has one "strong decoupling" state with its value of decoupling index changed from -0.380824-(-0.250250). The increased use of plastic film has resulted in increased agricultural pollution. The research results show that growing with the development of Chengdu's agricultural economy, the use of pesticides and fertilizers has been decreasing. Chengdu's agricultural economy and agricultural pollution were found to have a healthy and coordinated development. The development of agricultural economy has not caused much damage to the agricultural environment. In addition, with the decline of agricultural pollution, public health problems will also be reduced.